This application guide details the performance, throughput, and virus clearance capabilities of the Sartorius Virosart HF virus filter for mAbs and…
The Virosart HF virus filter family utilizes an asymmetric polyether-sulfone (PES) hollow-fiber membrane with a nominal pore size of 20 nm. It is designed for the virus filtration of monoclonal antibodies, antibody fragments, and small recombinant proteins towards the end of the purification process. The membrane features a funnel-like pore-size gradient and is surface modified with a hydrogel-forming, low binding polymer to ensure robust retention of small viruses while maintaining high product transmission.
| Membrane Type | Asymmetric polyether-sulfone (PES) hollow-fiber membrane |
|---|---|
| Nominal Pore Size | 20 nm |
| Target Product Size | < 150 kD |
| Water Flow Rate Test Pressure | 2.0 bar | 30 psi |
| Integrity Test Pressure | 4.5 bar | 65.25 psi |
| Integrity Test Duration | 5 min |
| Release Criteria Virus | Pseudomonas aeruginosa bacteriophage PP7 |
The main application is the virus filtration of monoclonal antibodies, antibody fragments, or small recombinant proteins towards the end of the purification process.
The membrane is surface modified with a hydrogel-forming, low binding polymer to reduce the adsorption of proteins and other potentially fouling species.
The release criteria is the retention of Pseudomonas aeruginosa bacteriophage PP7, which is used as a standard model virus for small viruses.