Leica SP5 2-Photon/Confocal System User Manual

This document is the user manual of Leica SP5 2-PHOTON/CONFOCAL SYSTEM. The system includes pulsed laser, cooler, tunable laser, visible light lasers, scanner, microscope, detector box, temperature controller and other components.

Brand: Leica
Category: Other
Document type: User Manual
Language: English
Pages: 34
File format: PDF
File size: 6.3 MB
Published: 12 October, 2023
Updated: 12 October, 2023
Model: SP5
MD5 checksum: D8AE9A224180C12768D4C11DF5ACCE86
PDF: Leica SP5 2-Photon/Confocal System User Manual PDF

About this manual

This manual describes the components of the Leica SP5 2-Photon/Confocal System, including the infrared laser, chiller, tandem scanner, confocal detectors, and various stage adapters. It details the setup for both 2-photon imaging using the Chameleon Ultra II laser and confocal imaging using visible light lasers such as Argon and HeNe.

What's inside

Components of the Leica SP5 System
Starting up the Leica SP5 system
Stage and objective adapters
Switching on the system
LAS AF acquisition software
Suitable dyes for Multi-Photon
Filter configuration

Specifications

Tunable excitation range	680-1080 nm
Pulse width	140 fs
Peak power	> 3.3 W
Resonant scanner speed	8000 Hz
Piezo travel distance	300m

Frequently asked questions

What is the speed of the resonant scanner?

The resonant scanner operates at a speed of 8000 Hz, or 16000 Hz in bidirectional mode.

What is the recommended stage adapter for live imaging?

The 'piezo' galvanometric actuator is recommended for live imaging.

Download PDF (6.3 MB)