Agilent BioSeparation Selection Guide

About this manual

This document serves as a selection guide for Agilent products designed for the entire workflow of bioseparation, from sample preparation to analysis. It highlights that these solutions are based on 40 years of technical experience and aim to provide reproducible and high-quality results for various applications, such as characterizing monoclonal antibodies or monitoring process purification. This manual is written in German.

What's inside

• Reversed Phase-HPLC-Biosaulen
• ZORBAX-Strategie tur die Reversed Phase-Methodenentwicklung
• Auswahl der Startersaule und Anfangsbedingungen
• Optimierung der Léslichkeit der Probe
• Erhéhen der Temperatur
• Optimierung des pH-Werts der mobilen Phase
• Trennungen von Proteinen und Peptiden mit LC/MS-Methoden
• Hohe Empfindlichkeit mit Kapillarsaulen
• Poroshell 300 Beschreibung
• Saulenspezifikationen

Specifications

Frequently asked questions

Which column is recommended for peptides and proteins with low molecular weight?

The StableBond 300SB-C8 column is the first choice for separations of peptides and proteins.

Can Poroshell 300Extend-C18 be used at high pH values?

Yes, the Poroshell 300Extend-C18 column can be used for special separations at pH 2 - 10.

What organic modifier is recommended for LC/MS separations instead of TFA?

Formic acid (Ameisensaure) or acetic acid (Essigsaure) should be used instead of TFA.