This quick reference guide provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast QPCR Master Mix with the Stratagene Mx3000P and Mx3005P QPCR Systems
This document serves as a quick reference guide for the Stratagene Brilliant II Ultra-Fast QPCR Master Mix when used with Agilent Mx3000P and Mx3005P QPCR Systems. It provides an optimized protocol for preparing reactions, including instructions on diluting the reference dye and combining reagent components such as water, master mix, probes, primers, and DNA templates.
| Reference Dye Dilution | 1:500 using nuclease-free PCR-grade water |
|---|---|
| Final Reaction Volume | 20 ul |
| Probe Concentration Range | 150-600 nM |
| Primer Concentration Range | 200-600 nM |
| Genomic DNA Quantity Range | 5 pg—100 ng |
| cDNA Quantity Range | 0.1 pg— 100 ng |
| Denaturation Step Duration | 5 seconds (optimized up to 20 seconds) |
The reference dye must be diluted 1:500 using nuclease-free PCR-grade water.
The protocol specifies bringing the final reaction volume to 20 ul.