This document describes the sample preparation methods and features of Agilent Proteomics Reagents for LC/MS products. These reagents are directly compatible with LC/MS methods and do not require any additional sample preparation or purifications. Specific products include Proteomics Grade Tryspin, FFPE Protein Extraction Solution, and PPS Silent Surfactant.
Agilent offers a suite of reagents designed for sample preparation in proteomics applications such as biomarker discovery, protein characterization, and shotgun proteomics. These products are directly compatible with LC/MS methods without requiring additional purification steps.
The document details three specific reagents: Proteomics Grade Trypsin, which is highly pure to prevent autolysis; FFPE Protein Extraction Solution, a detergent-free method for isolating proteins from formalin-fixed paraffin-embedded tissue that preserves immunological epitopes; and PPS Silent Surfactant, an acid-cleavable reagent designed to solubilize hydrophobic proteins without interfering with mass spectrometry analysis.
These reagents are used for sample preparation in proteomics applications such as biomarker discovery, protein characterization, and shotgun proteomics.
No, these reagents are directly compatible for use with LC/MS methods and do not require any additional sample preparation or purifications.