The use of short, small-particle columns allows for fast and efficient separations. The smaller particle size (3.5µm) provides similar separation efficiency to 5µm particles in columns that are twice as long. Increasing the flow rate on the 3.5µm, 75 mm column further decreases the run-time. The efficiencies achieved are nearly equivalent to those of the 5µm, 150 mm column run at a slower flow rate. This application is focused on agrichemicals.
The document presents an application using Agilent Technologies' ZORBAX SB-C8 columns to separate a herbicide mixture. It highlights that smaller particle size (3.5µm) allows for high-speed, high-resolution separation in less than 8 minutes compared to longer columns with larger particles.
The note provides specific chromatographic conditions and results, showing that increased flow rates on shorter columns further decrease run times while maintaining efficiencies nearly equivalent to those of standard 150 mm columns.
The short column has a length of 75 mm and an inner diameter of 4.6 mm.
The separation is completed in less than 8 minutes.
The standard column is 4.6 mm by 150 mm with a 5 µm particle size, while the short version uses 3.5 µm particles.