This document introduces the Agilent large-volume injection learning program, which introduces three large-volume injection methods, Hot Splitless, Cold Splitless and Solvent Blowing.
This document serves as a training guide for researchers using the Agilent Multimode Inlet (MMI) to perform Large Volume Injections (LVI). It explains the benefits of LVI over standard injection techniques, such as improved signal-to-noise ratios and reduced solvent effects. The guide details three specific injection modes: Hot Splitless, Cold Splitless, and Solvent Vent, highlighting their operational principles and advantages for different analyte types. This manual is written in German.
While standard methods typically process one or two microliters, LVI can allow injection volumes ranging from a few microliters up to one milliliter or more.
The training program uses a constant flow column mode with an inlet temperature of 250 °C, an initial time of 5 minutes, and a rate of 10 °C/Min.