Agilent 5188-2749 Protein In-Gel Tryptic Digestion Kit Instructions

About this manual

The Agilent Protein In-Gel Tryptic Digestion Kit (part number 5188-2749) provides a complete set of reagents for approximately 150 in-gel digestions. The kit includes modified trypsin, destaining buffers, digestion buffers, reduction reagents (TCEP), and alkylation reagents (IAA). It is designed to function with a wide range of protein band concentrations to produce complete and accurate digests for mass spectrometric analysis.

What's inside

• Kit Contents
• Storage
• Hazard Classifications
• Procedure Summary
• Important Product Information
• Additional Materials Required
• Material Preparation
• Protocol for In-Gel Digest

Frequently asked questions

How many digestions does the kit provide?

The kit includes sufficient reagents for approximately 150 in-gel digestions.

What type of stained gels is this kit designed for?

The kit is designed for colloidal Coomassie or fluorescent dye-stained acrylamide gel slices.

What are the common trypsin fragments used as internal standards?

The most common tryptic fragments have masses of 842.51 (m/z, M+ H) and 2211.10 (m/z, M + H).