This document is the Cell Proliferation Assay Kit Instruction Manual, which introduces the kit composition, storage conditions, and usage methods.
The Agilent Cell Proliferation Assay Kit is a system designed for accurately quantitating the number of cells in a culture, including both adherent and suspension cells. It can also be used to measure double-stranded DNA in samples of plasmid, lambda, or genomic DNA. The assay works by lysing cells and incubating the lysate with a dye that fluoresces when bound to DNA; fluorescence intensity is then measured using a standard fluorometer.
| Dye reagent concentrate quantity | 0.1 ml |
|---|---|
| Cell lysis buffer quantity | 100 ml |
| Assay capacity per kit | 1000 microassays in 96-well plates |
| Cell lysis buffer storage condition | 4°C |
The recommended volume is 100 µl per well.
The excitation wavelength is 355 nm, and the emission wavelength is 460 nm.
No, it is for research use only and not for use in diagnostic procedures.