SureSelectXT Target Enrichment System is a research-use-only product for SOLiD 4 Multiplexed Sequencing Protocol.
This document provides a detailed protocol for enriching targeted regions of the genome from repetitive sequences and unrelated research focus areas. It is specifically designed for use with Biotinylated RNA oligomer libraries, also known as Bait.
The guide covers essential steps including sample preparation, hybridization, addition of barcode tags via post-hybridization amplification, and reference information regarding reagent kits and equipment combinations.
The guide describes procedures to capture genomic regions of interest using Biotinylated RNA oligomer libraries, or Bait, to enrich targeted regions from repetitive sequences.
Users must generally follow Biosafety Level 1 (BL1) safety rules when working in the laboratory.
After briefly mixing on a vortex mixer, the tube must be spun in a centrifuge for 5 to 10 seconds.