Bio‑Rad DCode Universal Mutation Detection System User Manual

About this manual

The DCode Universal Mutation Detection System is a vertical electrophoresis instrument designed for mutation analysis methods such as DGGE, CDGE, TTGE, SSCP, PTT, and Heteroduplex Analysis. The manual covers safety guidelines, system components, and detailed procedures for preparing reagents, casting gels, and running experiments.

Key sections include general safety information, a step‑by‑step guide to system setup, operation of the temperature controller, and troubleshooting tips. Warranty terms, specifications, and a parts list are also provided.

What's inside

• General Safety Information
• Introduction to Mutation Detection Technology
• Product Description and Packing List
• System Components and Accessories
• Denaturing Gradient Gel Electrophoresis (DGGE, CDGE, TTGE)
• Single‑Stranded Conformational Polymorphism (SSCP)
• Protein Truncation Test (PTT)
• Heteroduplex Analysis
• Troubleshooting Guide
• Maintenance

Specifications

Frequently asked questions

What are the maximum voltage and power limits for the instrument?

The system can operate up to 500 VDC and a maximum power of 50 watts.

How does the safety interlock work on the DCode system?

When the lid is removed, the DC current to the cell is automatically broken, preventing operation.