The TC-5000 is a user-friendly and reliable device for temperature control and heating experiments. This manual provides detailed information on the installation, operation, and maintenance of the equipment.
This manual covers all aspects of the TC-5000 gradient thermal cycler, including safety precautions, unpacking, installation, and connection details. It also describes the instrument’s operating principles, programming options, and routine maintenance procedures.
Additional sections provide troubleshooting guidance, a list of accessories and replacement parts, and frequently asked questions to help users get the most out of the device.
| Temperature range | 4 °C – 99 °C |
|---|---|
| Ambient operating temperature | +5 °C to +40 °C |
| Maximum altitude | 2000 m |
| Power supply options | 220‑240 V 50/60 Hz or 110‑120 V 50/60 Hz |
| Safety feature | Over‑temperature cut‑out |
| Heating‑rate degradation at voltage extremes | ≈ 8 % |
The Hot Start step pauses the machine at a specific temperature, typically around 70 °C, after the initial template denaturation.
Incremental timing and temperature increase or decrease the time or temperature stepwise over the number of cycles in a stage.
Gradient blocks allow a temperature step to vary across the block so each column reaches a different temperature.
A temperature gradient lets users optimise annealing temperatures for primers, testing many temperatures in a single run.