This application note describes how to use the Turner BioSystems Modulus multifunction photometer and Quant-iT DNA HS assay kit to quantify dsDNA.
The document describes a workflow that combines the Turner BioSystems Modulus multifunction light meter with the Quant‑iT DNA HS assay kit to provide precise quantification of double‑stranded DNA in small volumes. It outlines the required hardware, reagents, and step‑by‑step procedures for sample preparation, instrument setup, calibration, and analysis.
Key points include reagent dilution (1:200), avoidance of glass containers, calibration using five standard samples, and reading results in ng/µl on the instrument display. Contact information for both Molecular Probes, Inc. and Turner BioSystems is also provided. This manual is written in Chinese.
| Instrument part number | 9200-000 or 9200-002 |
|---|---|
| Blue fluorescence excitation module part number | 9200-040 |
| Detection volume | 210 µl |
| Linear range | 5‑100 ng |
| Standard sample volume | 200 µl |
A sample volume of 210 µl is used, providing a linear range of 5‑100 ng of dsDNA.
Dilute the concentrated dye 1:200 with Quant‑iT DNA HS buffer, use only plastic containers, and keep the solution protected from light.
Five standard samples are recommended for calibration, with a zero‑concentration sample used as a blank.