This document provides information on the components and usage of the Version 02152011 Plasmid Mini Kit. It also highlights the features and advantages of extracting plasmid DNA.
The Plasmid Mini Kit (catalog No. CW0511) from CW Biotech offers a fast, alkaline‑lysis method to isolate high‑quality plasmid DNA from 1‑5 ml bacterial cultures. The kit includes buffers, spin columns, and collection tubes, allowing yields up to 30 µg of plasmid DNA without phenol extraction or ethanol precipitation.
The manual lists all kit components, outlines safety and handling precautions, and details the complete workflow: cell lysis with Buffer P1 (RNase A added), neutralization with Buffer P2, precipitation with Buffer N3, column binding, washing, and elution with Buffer EB. It also provides tips for troubleshooting, storage conditions, and required auxiliary reagents such as anhydrous ethanol. This manual is written in Chinese.
| Kit Size | 100 µg / 200 µg |
|---|---|
| Buffer P1 volume | 30 ml (100 µg) or 60 ml (200 µg) |
| Buffer P2 volume | 30 ml (100 µg) or 60 ml (200 µg) |
| Buffer N3 volume | 40 ml (100 µg) or 80 ml (200 µg) |
Add the RNase A supplied in the kit to Buffer P1, mix well, and store at 2‑8 °C.
Warm the buffer at 37 °C in a water bath for a few minutes to dissolve the crystals.
Store the eluted plasmid DNA at –20 °C.