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- Last added
- 2012-03-13
Featured Plasmid manuals
CW Biotech CW0511 Plasmid Mini Kit User Manual
The Plasmid Mini Kit (catalog No. CW0511) from CW Biotech offers a fast, alkaline‑lysis method to isolate high‑quality plasmid DNA from 1‑5 ml bacterial cultures. The kit includes buffers, spin columns, and collection tubes, allowing yields up to 30 µg of plasmid DNA without phenol extraction or ethanol precipitation.
The manual lists all kit components, outlines safety and handling precautions, and details the complete workflow: cell lysis with Buffer P1 (RNase A added), neutralization with Buffer P2, precipitation with Buffer N3, column binding, washing, and elution with Buffer EB. It also provides tips for troubleshooting, storage conditions, and required auxiliary reagents such as anhydrous ethanol. This manual is written in Chinese.
Covers: Product Introduction · Components List · Precautions · Procedure Steps · Ordering Information
Latest Plasmid manuals
CW Biotech CW0511 Plasmid Mini Kit User Manual
User Manual 2 pages · 239 KB · ZHThe CW Biotech CW0511 Plasmid Mini Kit User Manual provides step‑by‑step instructions, component list, and usage notes for extracting plasmid DNA.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Plasmid manuals
How should Buffer P1 be prepared before use?
Add the RNase A supplied in the kit to Buffer P1, mix well, and store at 2‑8 °C.
What to do if Buffer P2 or Buffer N3 shows crystals or precipitate?
Warm the buffer at 37 °C in a water bath for a few minutes to dissolve the crystals.