This kit is used to quantify GDH in serum, plasma and other biological fluids of rats.
About this manual
This manual provides detailed instructions for the LUscn Life Science rat Glutamate Dehydrogenase (GDH) ELISA kit. It lists the supplied reagents, required equipment, storage conditions, and the assay principle based on a double‑antibody sandwich ELISA.
Step‑by‑step procedures cover sample collection, reagent preparation, plate loading, washing, substrate development, and calculation of GDH concentrations. The document also includes detection limits, specificity information, and safety warnings. This manual is written in Chinese.
What's inside
- Product Information
- Intended Application
- Reagents Provided
- Equipment Required
- Storage and Stability
- Assay Principle
- Sample Collection and Storage
- Reagent Preparation
- Assay Procedure
- Washing Procedure
Specifications
| Product number | E0293Ra |
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| Detection range | 0.312 U/L – 20 U/L |
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| Lower limit of detection | 0.076 U/L |
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| Short‑term storage temperature | 4 °C |
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| Long‑term storage temperature | -20 °C |
Frequently asked questions
What is the detection range of the rat GDH ELISA kit?
The kit can detect GDH concentrations from 0.312 U/L up to 20 U/L.
How should the kit reagents be stored?
Short‑term storage is at 4 °C, while long‑term storage should be at –20 °C, protected from moisture.
What is the lower limit of detection for GDH?
The lower limit of detection is 0.076 U/L.
What equipment is required to read the assay results?
A microplate reader with a 450 ± 10 nm filter is required.