This kit can be used for quantitative determination of Apo-B100 in rat serum, plasma, cell culture supernatant or other related biological fluids.
About this manual
The rat Apo‑B100 ELISA immunoassay kit is designed for quantitative measurement of Apo‑B100 in various biological fluids such as serum, plasma, and cell culture supernatants. The kit provides a detection range of 78 ng/mL to 500 ng/mL with a lower detection limit of 1.95 ng/mL and is specific for natural or recombinant Apo‑B100 without cross‑reactivity to related proteins.
The kit includes a 96‑well assay plate, freeze‑dried standards, sample diluent, biotin‑labeled antibody, HRP‑avidin, substrate, wash buffer, and stop solution. The manual details sample collection, storage conditions, reagent preparation, step‑by‑step assay procedure, washing methods, and data analysis, along with important precautions to ensure reliable results. This manual is written in Chinese.
What's inside
- Detection Range and Sensitivity
- Reagents and Kit Components
- Sample Collection and Storage
- Assay Procedure
- Washing Methods
- Data Analysis and Calculation
- Precautions and Tips
Specifications
| Detection range | 78 ng/mL – 500 ng/mL |
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| Lower detection limit | 1.95 ng/mL |
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| Specificity | No cross‑reaction with other related proteins |
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| Shelf life | 6 months |
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| Storage temperature | -20 °C for long‑term, 2‑8 °C for frequent use |
Frequently asked questions
What is the detection range of the rat Apo‑B100 ELISA kit?
The kit can detect Apo‑B100 concentrations from 78 ng/mL up to 500 ng/mL.
What is the lower detection limit of the kit?
The lower detection limit is 1.95 ng/mL.
Does the kit cross‑react with other proteins?
No, the kit does not cross‑react with other related proteins.