This kit is used for the quantitative determination of INS in rat serum, plasma or other relevant biological fluids.
The USCNK E0448Ra kit provides a quantitative ELISA method for measuring insulin (INS) in rat serum, plasma, or tissue extracts. The assay is based on a sandwich format using coated 96‑well plates and a TMB substrate, with absorbance read at 450 nm.
The kit includes a 96‑well plate, coated and uncoated, reagents such as standards, detection antibodies, TMB substrate, and stop solution. The manual details sample collection, storage conditions, preparation of reagents, the step‑by‑step assay protocol, washing procedures, and how to calculate concentrations from the calibration curve. This manual is written in Chinese.
| Plate format | 96‑well plate |
|---|---|
| Reading wavelength | 450 nm (plus 10 nm filter) |
| Detection range | 0.001–3.000 OD |
| Limit of detection | 0.32 mluL |
Measure the absorbance at 450 nm (with a 10 nm filter).
Collect serum, let it clot for 30 min at room temperature, centrifuge at 1000 × g for 20 min, and store the supernatant at –20 °C or –80 °C.
The minimum detectable concentration is 0.32 mluL.