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Featured rat胰岛素(INS)酶联免疫吸附测定试剂盒 manuals
USCNK E0448Ra Rat Insulin ELISA Kit User Manual
The USCNK E0448Ra kit provides a quantitative ELISA method for measuring insulin (INS) in rat serum, plasma, or tissue extracts. The assay is based on a sandwich format using coated 96‑well plates and a TMB substrate, with absorbance read at 450 nm.
The kit includes a 96‑well plate, coated and uncoated, reagents such as standards, detection antibodies, TMB substrate, and stop solution. The manual details sample collection, storage conditions, preparation of reagents, the step‑by‑step assay protocol, washing procedures, and how to calculate concentrations from the calibration curve. This manual is written in Chinese.
Covers: Assay Principle · Reagents and Materials · Sample Collection and Storage · Assay Procedure · Washing Steps · Data Analysis and Calibration Curve
Latest rat胰岛素(INS)酶联免疫吸附测定试剂盒 manuals
USCNK E0448Ra Rat Insulin ELISA Kit User Manual
User Manual 5 pages · 405 KB · ZHThis manual explains how to use the USCNK rat insulin ELISA kit, covering reagents, sample handling, assay steps, and data analysis.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in rat胰岛素(INS)酶联免疫吸附测定试剂盒 manuals
What wavelength should be used to read the ELISA results?
Measure the absorbance at 450 nm (with a 10 nm filter).
How should serum samples be stored before testing?
Collect serum, let it clot for 30 min at room temperature, centrifuge at 1000 × g for 20 min, and store the supernatant at –20 °C or –80 °C.