LUscn E0029Ra Rat E-Selectin ELISA Kit User Manual

This kit is used to quantitatively measure the E-selectin content in rat serum, plasma or other related biological fluids.


LUscn E0029Ra Rat E-Selectin ELISA Kit User Manual - cover page
Brand
Rate
Category
Drugs
Document type
User Manual
Language
Chinese
Pages
5
File format
PDF
File size
293 KB
Published
23 January, 2012
Updated
01 October, 2023
MD5 checksum
A9ABA13F03F23CF3F368BC6590EAC91D

About this manual

The LUscn rat E‑Selectin ELISA kit (product number E0029Ra, 96‑well format) is designed for quantitative measurement of E‑Selectin in rat serum, plasma or other biological fluids using a double‑antibody sandwich ELISA. The kit includes pre‑coated plates, standards, detection reagents, TMB substrate and stop solution, and requires a microplate reader with a 450 ± 10 nm filter.

The manual details sample collection, reagent preparation, step‑by‑step assay procedure, washing methods, and data analysis. It also provides storage conditions, shelf life, detection range, limit of detection, and safety warnings to ensure reliable results. This manual is written in Chinese.

What's inside

  • Product Information
  • Intended Application
  • Reagent Storage and Shelf Life
  • Experimental Principle
  • Sample Collection and Preservation
  • Reagent Preparation
  • Assay Procedure
  • Washing Methods
  • Data Calculation
  • Detection Range and Sensitivity

Specifications

Product numberE0029Ra
Detection range39 pg/mL – 2,500 pg/mL
Minimum detection limit17.6 pg/mL
Storage temperature-20 °C

Frequently asked questions

What is the detection range of the rat E‑Selectin ELISA kit?

The assay can detect E‑Selectin concentrations from 39 pg/mL up to 2,500 pg/mL.

How should the kit reagents be stored?

All reagents should be stored at –20 °C; the coated plate must be sealed with desiccant and kept at –20 °C after opening.

What type of microplate reader is required?

A microplate reader equipped with a 450 ± 10 nm filter is required for measuring absorbance.

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