This document describes the usage of PowerPlex® Matrix Standards 3100 kit, including the generation of spectral calibration files and amplification of sample DNA.
The PowerPlex Matrix Standards 3100 kit provides four fluorescently labeled DNA fragments (JOE160, FL250, TMR330, CXR350) for generating spectral calibration files on the ABI PRISM 3100 genetic analyzer. The manual describes the kit components, storage requirements, and the overall workflow for creating a calibration matrix.
Step‑by‑step procedures cover matrix sample preparation, instrument preparation, spectral run mode configuration, calibration execution, result evaluation, and data analysis using GeneScan and PowerTyper macros. Troubleshooting guidance and related product information are also included. This manual is written in Chinese.
| Fluorescent fragments | JOE160, FL250, TMR330, CXR350 |
|---|---|
| Tube volume per fragment | 1.25 ml |
| Storage temperature | -20 °C |
| Light sensitivity | Must be kept in darkness |
| Calibration Q‑value requirement | > 0.95 |
| Calibration C‑value range | 6.0 – 9.0 |