PowerPlex ®Y System is a PCR amplification kit for detecting STR regions of the Y chromosome. This system can be used for parentage testing and kinship analysis.
The PowerPlex Y System enables multiplex amplification of 12 Y‑STR loci with three‑color detection. The kit provides all reagents needed for PCR, post‑PCR processing and electrophoretic analysis, and includes guidance for optimizing conditions on various genetic analysis instruments.
The document details reagent composition, storage requirements, safety warnings, and step‑by‑step procedures for setting up PCR reactions, handling samples, and interpreting results. Compatibility with specific ABI PRISM and Applied Biosystems instruments is also described. This manual is written in Chinese.
| Reactions per DC6760 kit | 200 reactions |
|---|---|
| Reactions per DC6761 kit | 50 reactions |
| Number of Y‑STR loci amplified | 12 loci |
| Compatible instruments | ABI PRISM 310, 3100, 3100‑Avant, 3130, 3130xl, ABI PRISM 377 |
| Recommended storage temperature | -20 °C |
The system is compatible with ABI PRISM 310, 3100, 3100‑Avant, 3130, 3130xl and ABI PRISM 377 DNA sequencers.
The DC6760 kit provides enough reagents for 200 reactions of 25 µl each.
All components should be stored at –20 °C, and light‑sensitive reagents must be protected from light.