E0543r IgM ELISA kit is a quantitative assay for IgM in rat serum, plasma, cell culture supernatant or other relevant liquids. The kit uses a double antibody sandwich enzyme-linked immunosorbent assay. Purified antibodies are coated on microwell plates to form a solid-phase antibody. IgM, biotinylated anti-rat IgM antibody, HRP-labeled affinity reagent are added to the wells coated with single antibodies in sequence, and then thoroughly washed with TMB substrate. TMB is converted to blue by peroxidase catalysis and converted to the final yellow by acid. The color depth is positively correlated with the IgM content in the sample. The absorbance (OD value) is measured at 450nm using an enzyme spectrophotometer and the sample concentration is calculated.
