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Takara HB101 Electro-Cells User Manual
The Takara HB101 Electro-Cells are high‑efficiency competent E. coli cells designed for gene‑recombination experiments. They contain the genotype SUDE44, ADCR‑An1T, recA413, among others, and are supplied as 50 µL aliquots (10 × HB101) with control DNA (PBR322, 0.01 ng/µL). The cells are stored at –80 °C and must be thawed on ice before use.
The manual describes the electroporation protocol: thaw the Electro‑Cell, add 1–2 µL DNA (desalting if needed), mix in a pre‑chilled 0.1 cm cuvette, apply a high‑voltage pulse, immediately add 1 mL SOC medium, incubate at 37 °C for 1 h with shaking, then plate on agar. Precautions include dry‑ice transport, avoiding refreezing after thaw, and limits on DNA size and amount for optimal efficiency. This manual is written in Chinese.
TAKARA HB101 specifications
| Cell concentration | 1 ~ 2 × 10^10 cells/mL |
|---|---|
| Recommended DNA amount for transformation | 10 pg plasmid DNA |
| Typical transformant yield | ≈5 × 10^8 transformants per 10 pg DNA |
All TAKARA HB101 documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
How should the Electro‑Cell be stored before use?
Store the Electro‑Cell at –80 °C; after thawing it must not be refrozen.
What transport condition is required for the Electro‑Cell?
The Electro‑Cell must be transported using dry ice.