Key facts
- Brand
- TAKARA
- Model
- HST08
- Documents
- 2
- Languages
- ZH
- Last added
- 2011-12-23
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Takara HST08 Premium Competent Cells Manual
The HST08 Premium competent cells are a high‑efficiency strain supplied by Takara for cloning applications, especially when target DNA is present at low concentrations. The product includes 100 µl aliquots (10 tubes) and a control pUC19 DNA (0.1 ng) for reference.
The manual details thawing, mixing with up to 10 ng of DNA, a 30‑minute ice incubation, a 45‑60 second heat‑shock at 42 °C, and recovery in SOC medium. It also lists storage at –80 °C, recommended tube types, and precautions to maintain transformation efficiency. This manual is written in Chinese.
TAKARA HST08 specifications
| Packaging | 100 µl × 10 tubes |
|---|---|
| Control DNA | pUC19, 0.1 ng per tube (10 × 1) |
| Cell Concentration | 1–2 × 10³ cells/µl |
| Typical Transformation Efficiency | > 1 × 10⁸ transformants per µg pUC19 |
| Heat‑Shock Condition | 42 °C for 45–60 seconds |
| SOC Medium Composition | 2% Bacto tryptone, 0.5% Bacto yeast extract, 10 mM NaCl, 2.5 mM K₂HPO₄, 10 mM MgSO₄, 10 mM MgCl₂, 20 mM Glucose |
All TAKARA HST08 documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What heat‑shock time and temperature are recommended for transformation?
After adding DNA, incubate the mixture at 42 °C for 45–60 seconds.
What is the composition of the SOC recovery medium?
SOC contains 2% Bacto tryptone, 0.5% Bacto yeast extract, 10 mM NaCl, 2.5 mM K₂HPO₄, 10 mM MgSO₄, 10 mM MgCl₂, and 20 mM glucose.