Key facts
- Manuals
- 5
- Languages
- EN · ZH
- Last added
- 2026-10-30
Featured cytiva manuals

Cytiva Histidine-Tagged Protein Purification Selection Guide
This guide outlines Cytiva’s chromatography media for purifying histidine-tagged proteins from prokaryotic and eukaryotic systems. Products include precharged nickel and cobalt resins, magnetic beads, and prepacked columns for various applications. The guide helps users select based on expression system, purification scale, and equipment compatibility.
Detailed product categories are presented with specifications like binding capacity, pack sizes, and compatibility with systems such as ÄKTA™. Formats include 96-well plates for high-throughput screening, spin columns for minipreps, and gravity-flow columns for batch purification.
Covers: Ni Sepharose High Performance · Ni Sepharose 6 Fast Flow · His Mag Sepharose Ni · TALON Superflow · Ni Sepharose excel · His Mag Sepharose excel

Cytiva ÄKTA avant Protein Liquid Chromatography System Product Overview
The Cytiva ÄKTA avant is a fully automated intelligent liquid chromatography system designed for method exploration and purification process optimization. It offers two models: the ÄKTA avant 25 with a maximum flow rate of 25 ml/min, and the ÄKTA avant 150 with a maximum flow rate of 150 ml/min. Both systems share the same hardware architecture but differ in pressure parameters to suit different sample scales.
The system is controlled by UNIC ORN™ 6 software, which includes tools for experimental design (DoE), automatic buffer preparation via BufferPro, and integrated semi-conductor refrigerated fraction collectors. Key features include an interactive touch display, automated air bubble detection, and a modular flow path design that allows easy access to valves, detectors, and columns. This manual is written in Chinese.
Covers: System Overview · Pumps (System and Sample) · Valves and Flow Control · Mixing Chamber · Sensors and Detectors · Fraction Collector

Cytiva MabSelect PrismA Resin Transition Guide
This document provides a guideline for transitioning from MabSelect SuRe™ LX or MabSelect SuRe™ resins to the next-generation MabSelect™ PrismA resin for the capture of monoclonal antibodies and Fc-containing recombinant proteins. MabSelect PrismA features a rigid, high-flow agarose base matrix and an alkaline-stabilized ligand derived from protein A, offering increased capacity and alkaline stability compared to its predecessors.
Key considerations for a successful transition include upgrading immunoassays to quantify leached ligand, adjusting column packing methods to adapt to updated pressure-flow properties, addressing increased mass throughput in polishing steps, and implementing improved cleaning and sanitization protocols due to higher alkaline stability.
Covers: Introduction · Resin Characteristics · Ligand Manufacture · Elution Conditions and Specificity · Toxicity Data · Ligand Leaching

Cytiva IN Carta SINAP User Manual
The Cytiva IN Carta SINAP software is a trainable segmentation module that utilizes both classic machine learning and deep learning to identify regions of interest in biological images. It allows researchers to extract quantitative information from diverse datasets, such as tissue, whole organisms, cells, and organelles, ensuring robust analysis by minimizing segmentation errors.
Covers: Important user information · Introduction · General user information · Launch the application · Create new protocol with deep learning analysis · Refining a pre-trained model
Latest cytiva manuals
Cytiva Histidine-Tagged Protein Purification Selection Guide
Product Leaflet 14 pages · 1.7 MB · ENCytiva's selection guide for histidine-tagged protein purification resins, including Ni Sepharose, TALON Superflow, and IMAC Sepharose formats.
Cytiva ÄKTA avant Protein Liquid Chromatography System Product Overview
Product Leaflet 12 pages · 2.4 MB · ZHThis document introduces the Cytiva ÄKTA avant, an automated intelligent liquid chromatography system for method exploration and purification optimization.
Cytiva MabSelect PrismA Resin Transition Guide
User Manual 14 pages · 292 KB · ENA guide for biopharmaceutical manufacturers on transitioning from MabSelect SuRe resins to the next-generation MabSelect PrismA affinity chromatography resin.
Cytiva IN Carta SINAP User Manual
User Manual 20 pages · 545 KB · ENThis manual provides instructions for using the Cytiva IN Carta SINAP software for deep learning-based image segmentation and analysis.
Cytiva Biacore T200 User Manual
User Manual 11 pages · 304 KB · ENUser guide for the Biacore T200 surface plasmon resonance instrument for real-time molecular interaction analysis.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in cytiva manuals
What are the benefits of Cytiva’s histidine-tagged protein purification products?
Flexible choice of uncharged or nickel/cobalt precharged media, high protein binding capacity with low leakage of metal ions, and range of formats for different applications.
From: Cytiva Histidine-Tagged Protein Purification Selection Guide
Which media are suitable for eukaryotic expression systems?
Ni Sepharose excel and His Mag Sepharose excel are designed for screening and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.
From: Cytiva Histidine-Tagged Protein Purification Selection Guide
What is the purpose of the ÄKTA avant system?
It is used for method exploration and purification process optimization.
From: Cytiva ÄKTA avant Protein Liquid Chromatography System Product Overview
What software controls the ÄKTA avant?
The system uses UNIC ORN™ 6 control software.
From: Cytiva ÄKTA avant Protein Liquid Chromatography System Product Overview
What is the main difference between MabSelect PrismA and MabSelect SuRe resins?
MabSelect PrismA features an optimized base matrix and ligand for increased capacity and alkaline stability compared to MabSelect SuRe.
Is the MabSelect PrismA ligand free of animal-derived components?
Yes, the MabSelect PrismA ligand is produced in E. coli and is free of animal-derived components.
