Cytiva's selection guide for histidine-tagged protein purification resins, including Ni Sepharose, TALON Superflow, and IMAC Sepharose formats.
This guide outlines Cytiva’s chromatography media for purifying histidine-tagged proteins from prokaryotic and eukaryotic systems. Products include precharged nickel and cobalt resins, magnetic beads, and prepacked columns for various applications. The guide helps users select based on expression system, purification scale, and equipment compatibility.
Detailed product categories are presented with specifications like binding capacity, pack sizes, and compatibility with systems such as ÄKTA™. Formats include 96-well plates for high-throughput screening, spin columns for minipreps, and gravity-flow columns for batch purification.
| Protein Binding Capacity | 40 mg/ml (Ni Sepharose HP) |
|---|---|
| Metal Ion | Cobalt (TALON Superflow) |
Flexible choice of uncharged or nickel/cobalt precharged media, high protein binding capacity with low leakage of metal ions, and range of formats for different applications.
Ni Sepharose excel and His Mag Sepharose excel are designed for screening and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.
TALON Superflow is a cobalt-based IMAC medium offering enhanced selectivity for histidine-tagged proteins, suitable for purification under native or denaturing conditions with lower imidazole concentrations.