Agilent Manuals (Others)

This article describes a method for increasing sample load in preparative liquid chromatography by using DMSO as the injection solvent.

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This paper mainly introduces the method of using Agilent Prep reverse phase column to realize the gradient separation of corticosteroids. Different diameter Agilent Prep reverse phase columns show nearly linear scale-up for the gradient separation of corticosteroids. With as much as 8 mg of total corticosteroid injected onto a 4.6 mm column and 320 mg onto a 30 mm column, the loadability of the columns is quite good.

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This compendium describes the applications of the Agilent LC/MSD TOF technology in the pharmaceutical industry, focusing on the advantages of high resolution, high accuracy, and wide dynamic range TOF mass spectrometry in drug development.

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This technical note describes some of the typical quality control tests and specifications for Agilent 2100 Bioanalyzer Protein 200 Plus LabChip Kits.

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This Application Note describes the general use of cell fluorescence assays on the Agilent 2100 bioanalyzer. The cell assay extension together with the cell fluorescence LabChip® kit allows both the analysis of pre-stained cells and the analysis of cells by on-chip staining. Cell assays on the 2100 bioanalyzer are based on simple flow cytometric analysis. The technical principles, as well as the typical workflow in sample and chip preparation are described here. A typical dye optimization experiment was performed and the appropriate dye concentration for a specific cell line determined. The cell fluorescence software is easy-to-use and allows different forms of data evaluation and result presentation. Using an example, data evaluation is explained in detail. Specific advantages of cell fluorescence analysis with the Agilent 2100 bioanalyzer are low cell and reagent consumption, ease-of-use, automated data acquisition and evaluation of up to six samples.

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This application is a tool to aid RNA data interpretation and identify features from total RNA electropherograms that reveal information about RNA sample quality.

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This paper introduces a method for the separation of basic drugs, such as ranitidine and paroxetine, based on hydrophilic interaction liquid chromatography (HILIC) and electrospray ionization mass spectrometry (MS/MS) detection. The method showed good linearity over the 0.5–100 ppb levels. A spiked serum was cleaned up by protein precipitation and an aliquot was directly injected into the HILIC/ESI-MS system. Recovery was found to be 98% for ranitidine and 79% for paroxetine at the 100 ppb level.

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Laboratories can improve the efficiency of their operations and ensure the accuracy of their data reporting, by automating the transfer of information between their chromatography data system (CDS) and their laboratory information management system (LIMS). In addition, the security of their chromatography data is enhanced when this automation is accomplished within the security framework established by regulatory guidelines such as the U.S. Food and Drug Administration’s ruling on electronic signatures and electronic records (21 CFR Part 11). This case study describes how Labtronics LimsLinkCDS enables laboratories to, smoothly and securely, integrate Agilent Cerity for Pharmaceutical QA/QC with any LIMS.

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This is an article about the automated method development of Agilent 1100 Series HPLC systems. The article introduces the use of ChromSword Auto software in Agilent 1100 Series HPLC systems and Agilent ChemStation software.

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This document introduces the automatic method development of Agilent 1100 Series HPLC systems, Agilent ChemStation software and ILS ChromSmart MD software.

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This is a document that introduces the features of the product.

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This Application Note describes the rapid development of a MRM method for triple quadrupole mass spectrometry, the rapid development of a liquid chromatography method for high throughput conditions with a 1.8 µm RRHT column, the use of the developed LC/MS MRM method for rapid determina- tion of a metabolic stability profile of a pharmaceutical drug, and the relative quantification of the acquired data to generate the metabolic stability profile.

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The Agilent PCR Preparation Workstation is a flexible workstation that provides high-throughput PCR preparation. Up to 150 microplates can be processed in one run (with tip changing). The plate processing time is approximately 5 hours for a 50 microplate run, depending on the protocol complexity.

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Analysis of Aminoglycoside Antibiotics

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This article introduces the application of the Agilent Technologies 6140 Single Quadrupole LC/MS High Throughput Mass Spectrometer. The Agilent 1200 Series Rapid Resolution Liquid Chromatography (RRLC) system is used to analyze the impurities of octyl dimethyl para-aminobenzoic acid (OD-PABA). The instrument has a high scan speed capability, which allows clear detection of the degradation product p-dimethylbenzoic acid. The background and usage of OD-PABA are also discussed in this article.

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Application Note Abstract Microarray-based gene expression hybridization is a powerful and proven technique for studying differential gene expression signatures in cancer. RNA extracted from freshly frozen tissues is optimal for microarray analysis; however, in many cases, formalin-fixed paraffi n-embedded (FFPE) tissues are the only samples available. To work with these diffi cult samples, Agilent has developed an FFPE sample protocol optimized for use with Agilent gene expression microarrays. To test the effectiveness of this protocol, total RNA was extracted from a quadruplicate set of colon tumor (adenocarcinoma), and matched normal colon FFPE and fresh frozen tissues. RNA purity and quality were determined, and the RNA was amplifi ed using a system in which a reverse transcription-generated cDNA library is subjected to limited PCR amplifi cation. The resulting cDNA target was chemically labeled. Analysis of the microarray results revealed a reduced sensitivity of detection for the FFPE-derived RNA samples; however, the lists of detected genes showed high overlap between the FFPE and frozen samples. Comparison of gene expression signals demonstrated excellent assay reproducibility between the technical repli- cates. Principal component analysis of array signals demonstrated clear separa- tion between the tumor and normal samples with grouping of replicate samples. Statistical analysis of the expression level variations between tumor and normal samples revealed that both the FFPE and frozen samples showed similar expression level changes.

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This document describes a one-color gene expression capability developed by Agilent Technologies, as well as the performance and advantages of this system.

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