Agilent Technologies Cell fluorescence assays on the Agilent 2100 bioanalyzer Application Note
Update: 01 October, 2023
This Application Note describes the general use of cell fluorescence assays on the Agilent 2100 bioanalyzer. The cell assay extension together with the cell fluorescence LabChip® kit allows both the analysis of pre-stained cells and the analysis of cells by on-chip staining. Cell assays on the 2100 bioanalyzer are based on simple flow cytometric analysis. The technical principles, as well as the typical workflow in sample and chip preparation are described here. A typical dye optimization experiment was performed and the appropriate dye concentration for a specific cell line determined. The cell fluorescence software is easy-to-use and allows different forms of data evaluation and result presentation. Using an example, data evaluation is explained in detail. Specific advantages of cell fluorescence analysis with the Agilent 2100 bioanalyzer are low cell and reagent consumption, ease-of-use, automated data acquisition and evaluation of up to six samples.
Brand: Agilent
File format: PDF
Size: 194 KB
MD5 Checksum: B619721680A7986517733DD13B1CF0B7
Publication date: 02 July, 2012
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PDF Link: Agilent Technologies Cell fluorescence assays on the Agilent 2100 bioanalyzer Application Note PDF