Agilent Manuals (Others)

This Application Note describes a high-speed, high-resolution capillary electrophoresis method of heparin and related impurities using highly concentrated buffer in a 25 µm bubble cell capillary. The method can be used to detect heparin and related impurities in a short time.

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This Application Note describes the rapid separation of metabolites generated from in-vitro experiments using the Agilent 1290 Infinity LC system. It also explains the fast acquisition of TOF mass spectra using Agilent 6530 Accurate-Mass Quadrupole Time-of-Flight LC/MS systems. The software-assisted identification of main metabolites from in-vitro experiments using Agilent MassHunter Metabolite Identification software is discussed. The generation of reports for the identified metabolites using Agilent MassHunter software is also covered.

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This Application Note demonstrates: The use of the Agilent 1200 Rapid Resolution LC (RRLC) system for high resolution separation of metabolites from an in-vitro metabolism experiment. The use of the Agilent 6520 QTOF mass spectrometer for the acquisition of data for computer assisted metabolite identification. The use of the Agilent MassHunter Metabolite identification software for highly productive identification of expected metabolites. The results of the Metabolite ID data analysis for expected metabolites of the pharmaceutical drug nefazodone

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This Application Note demonstrates: (1) The use of the Agilent 1200 Rapid Resolution LC (RRLC) system for high resolution separation of metabolites from an in-vitro metabolism experiment. (2) The use of the Agilent 6520 QTOF mass spectrometer for the acquisition of data for computer assisted metabolite identification. (3) The use of the Agilent MassHunter Metabolite identification software for highly productive identification of expected metabolites. (4) The results of the Metabolite ID data analysis for expected metabolites of the pharmaceutical drug nefazodone.

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Agilent has further improved the sample throughput capabilities of its 7500cx ICP-MS with Octopole Reaction System (ORS) using a newly configured Integrated Sample Introduction System–Discrete Sampling (ISIS-DS) accessory, and helium collision mode. Employing this new methodology, a complete suite of 30 or more elements can be analyzed in compliance with USEPA criteria (spectrum mode, three replicates, and sub-ppb MDLs) in approximately 75 seconds, sample to sample, with excellent removal of polyatomic interferences. Performance data showing stability, interference control, accuracy, precision, and washout are presented.

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This paper introduces a new method for environmental semivolatiles detection using a triple-axis detector (TAD) to achieve femtogram GC/MSD detection limits with improved sensitivity. The method uses liquid-solid extraction (LSE) with C18 disks or columns for sample extraction, 1 µL hot split-less injection for sample injection, and MS operating in full-scan mode. This method significantly improves the sensitivity to the femtogram level when measuring acids, bases, neutrals, and pesticides in drinking water and source water.

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This Application Note describes the nature of the electrostatic potential near the surface of a particle, called the zeta potential. It explains how the zeta potential is determined by measuring the velocity of the particles in an electric field. It also explains how the charge on the particle surface affects properties of suspensions and emulsions.

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Explains how the Agilent 7020 ZetaProbe can be used to control the stability of a colloid

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Definitive Quality Control of Titania Coatings with the Agilent 7020 ZetaProbe

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This abstract describes the application of the Agilent 6510 Quadrupole Time-of-Flight Mass Spectrometer (QTOF) for the analysis of pharmaceutical compounds in water.

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Rapid Analysis of High-Matrix Environmental Samples Using the Agilent 7500cx ICP-MS Application

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The method is based on the use of a mixed 119Sn-enriched spike and uses GC-MS coupled with electron impact ionization to simultaneously determine monobutyl, dibutyl, and tributyltin in water, sediments, and biota.

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The new Agilent 7500cx allows the user to achieve the full potential of ICP-MS for semiquantitative elemental screening of a wide range of sample types. Complex, unknown samples can be analyzed with better speed, accuracy, and data integrity than ever before, since all matrix interferences are removed in the Octopole Reaction System (ORS) using helium collision mode. Results are presented for three different certified reference materials.

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This Application Brief describes the use of an Agilent 6410 Triple Quadrupole Mass Spectrometer (QQQ) to analyze perfluorooctanoic acid (PFOA). A simple isocratic elution is carried out on a Rapid Resolution High Throughput C18 column (particle size 1.8 µm) with only water and methanol solvents containing 10 mM ammonium acetate. Elution time for standard dilutions of PFOA is only 2.3 minutes. Good linearity over more than 4 orders of magnitude, from 9 fg/µL to 150 pg/µL, is demonstrated with excellent peak area reproducibility of 5.5 % RSD at the 9 fg/µL level. The average peak-to-peak signal-to-noise (S/N) ratio at this level is 7.2. Sensitivity of surface water extracts is expected to be similar.

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This document describes an indoor air analysis method using thermal desorption/gas chromatography/mass spectroscopy (TD/GC/MS), and the creation of an Indoor Air Toxics Database. The database contains retention times and mass spectra for 171 volatile and semi-volatile organic compounds. With the use of the database and Agilent's Deconvolution Reporting Software (DRS), it is possible to identify these compounds within 2 to 3 minutes after TD/GC/MS analysis. The document also discusses the features and capabilities of DRS, as well as the analysis results of air samples from an office building and a carpet warehouse.

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This application note describes a method for analyzing pharmaceuticals in water using solid-phase extraction (SPE) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) in both positive and negative ion modes. Good linearity was observed for analytes from 1 pg to 1 ng on column. Repeatability from six injections of analytes at 5 pg on column showed RSDs below 15%, for all target compounds except for fluoxetine at 23%.

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This article introduces ways to improve the throughput of ICP-MS analysis of environmental samples, including using the 7500ce ICP-MS to optimize sample uptake and rinse time through various techniques, as well as using new rinse modes to improve productivity.

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