Agilent Technologies Hydrophilic Interaction Chromatography (HILIC) Separation of Basic Drugs using MS/MS Detection Application Note
Update: 30 September, 2023
This paper introduces a method for the separation of basic drugs, such as ranitidine and paroxetine, based on hydrophilic interaction liquid chromatography (HILIC) and electrospray ionization mass spectrometry (MS/MS) detection. The method showed good linearity over the 0.5–100 ppb levels. A spiked serum was cleaned up by protein precipitation and an aliquot was directly injected into the HILIC/ESI-MS system. Recovery was found to be 98% for ranitidine and 79% for paroxetine at the 100 ppb level.
Brand: Agilent
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Size: 87 KB
MD5 Checksum: 25BED0F4D70BFAD0BA1EA40F36FBA83A
Publication date: 02 July, 2012
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