Agilent Manuals (Others)

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This document describes the role of insulin in the human body and presents the results of a quality control experiment on insulin.

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This article describes a high-performance liquid chromatography analysis method for quality control of heparins. Heparins are mucopolysaccharides used to prevent thrombolysis in cases of hyperlipidemia, arteriosclerosis, blood transfusions, and operations. The analysis was conducted using an Agilent 1100 Series GPC-SEC system, which includes a vacuum degasser, thermostatted column compartment, refractive index detector, and other equipment. The results indicate that the method has high analytical accuracy and stability.

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The document describes an analysis method for dextran used in cosmetic formulations. The presence of impurities in dextran can be detected by using a chromatographic analysis method combining PL aquagel-OH mixed column and PL aquagel-OH 30 column. The ChemStation GPC data analysis software is used for data processing. The properties of dextran primarily depend on the molecular weight, molecular weight distribution, and impurities.

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This document describes the working principle and features of the Agilent 1100 Series purification system. The system allows for fractionation and re-analysis of compounds using the same instrument without any hardware modification. The software enables automated re-analysis of fractions, allowing for automated and unattended operation of the fractionation/re-analysis process. The document also provides details on the specific operation methods and conditions for fractionation and analysis using the system.

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This document describes how the Agilent 2100 Bioanalyzer replaces gel electrophoresis in prostate cancer research.

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This paper mainly studies the light-emitting polymer technology, describes the characteristics of light-emitting polymers, including molecular structure, solubility, and processing of thin films.

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This document introduces the conditions for analysis of anti-tumor drugs using the Agilent 1100 series HPLC systems.

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Electron capture negative chemical ionization mass spectrometry is used to analyze cannabinoids in biological matrices.

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The analysis of pesticides in water, foods, and soil is important worldwide. This document discusses the changes and regulations in environmental monitoring in the United States, Europe, Asia-Pacific region, and Latin America. It highlights the challenges and limitations of using gas chromatography/mass spectrometry (GC/MS) for pesticide detection, and the reliance on gas chromatography with an electron capture detector (GC/ECD). It also introduces the new 6890/5973 GC/MS system, which offers lower detection limits and can meet the current demands of environmental monitoring.

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Accurate mass measurements can be used to both confirm the presence of a compound and help identify unknowns. Obtaining accurate mea- surements using mass spectrometry has typically required highly skilled operators doing interactive instrument operation and data manipula- tion. This Application Note describes how using the Agilent LC/MSD TOF and new software for walk-up operation, chemists can get the accurate mass information they need easily and without much training.

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This application note describes the preparative isolation of corticosteroid degradation products using mass-based fraction collection.

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This application note describes the use of the Agilent 2100 bioanalyzer for stringent RNA quality control and the advantages of this method for high-resolution analysis of small and very small RNA samples.

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This application note describes how the attributes of Time of Flight and Trap mass spectrometry can be combined to provide a highly reliable identification for an unknown compound. Time of Flight mass spectrometry provides a very accurate mass for the parent compound, and Trap mass spectrometry provides fragmentation data; together they greatly diminish the number of candidate compounds, and increase the confidence in a confirmation.

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This article investigates the structure identification and confirmation of degradation products of the antibiotic drug amoxicillin. Accurate mass measurement and empirical formula confirmation for the compound ions and CID fragments were performed using ESI TOF.

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This document describes experimental modifications to inhibit ionization suppression when using LC-ESI-MS in negative ion mode for steroid estrogens and acidic herbicides. It introduces the problem of ionization suppression and methods to reduce its effects. The application of matrix clean-up in water samples to improve the apparent extraction efficiency of the analyte of interest when using LC-ESI-MS in negative ion mode is also discussed.

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The document describes the metabolism of drugs in the body and the impact of enzyme activity on the formation and excretion rate of metabolites. The CYP3A4 and CYP2D6 enzyme families are considered the most important in this activity. The document uses dextromethorphan as a probe for the activity of the CYP2D6 metabolic phenotype and describes the metabolism and excretion of the drug in different metabolic types of patients. Additionally, the document mentions that some individuals have multiple copies of the gene that produces the CYP2D6 enzymes, leading to high metabolic rates of many drugs in these individuals and rendering the drugs therapeutically ineffective.

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DuraGuard Columns: GC Columns with Built-In Protection

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This application note demonstrates the identification and confirmation of degradation products, which have emerged in a formulation trial of the antibiotic drug amoxicillin under non-optimal conditions.

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Agilent Reversed Phase Prep columns demonstrated both good loadability and good scalability for calcium channel blockers using gradient conditions. The columns also dis- played good resolution at all dimensions and allowed a linear scaleup from analytical to preparative dimensions

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