User manual for YC-A007-Luc-P 293T-Luc stable transfected cell line, covering culture, passaging, freezing, and luciferase assay protocols.
This document provides detailed instructions for handling and using the YC-A007-Luc-P 293T-Luc stable transfected cell line. It includes protocols for cell culture, passaging, freezing, and luciferase activity assays using the Dual-Luciferase® Reporter Assay System. The cell line expresses luciferase and puromycin resistance, suitable for gene expression and promoter activity studies.
The manual covers cell reception, thawing, subculturing, and freezing procedures, including specific steps for preparing media, using trypsin, and performing fluorescence detection with a BioTek SynergyLX instrument. This manual is written in Chinese.
| Cell Name | 293T-Luc |
|---|---|
| Cell Morphology | Epithelial, adherent |
| Fluorescence Resistance | Luciferase, Puro |
| Passaging Ratio | 1:4~1:6 |
| Culture Medium | 90%DMEM+10%FBS |
| Freezing Medium | 50%DMEM+40%FBS+10%DMSO |
Use the Dual-Luciferase® Reporter Assay System (Promega, Cat: E1910) and read luminescence on a BioTek SynergyLX instrument.
Passage cells at a ratio of 1:4 to 1:6, or adjust based on growth rate.