The UElandy YF® Click-iT EdU Imaging Kit manual provides reagent specifications, storage conditions, and step‑by‑step protocols for cell proliferation…
The YF® Click-iT EdU Imaging Kit from UElandy is a research reagent for detecting cell proliferation via EdU incorporation and click chemistry labeling. The kit includes EdU, azide‑conjugated fluorescent dyes (YF® 488/555/594/647A), reaction buffers, CuSO₄, and Hoechst 33342 for DNA counter‑staining.
The manual details storage requirements (‑20 °C, protected from light), recommended reagent concentrations, and a complete experimental workflow—from cell seeding and EdU labeling to fixation, permeabilization, click reaction, optional DNA staining, and imaging. Safety notes and troubleshooting tips are also provided. This manual is written in Chinese.
| EdU concentration | 10 mM |
|---|---|
| Azide volume per 20T kit | 4 µL |
| Fluorescence excitation/emission (YF® 488) | 495/519 nm |
| Fluorescence excitation/emission (YF® 555) | 555/565 nm |
| Shelf life after opening | up to 1 year |
Store the kit at –20 °C in the dark; the reagents remain stable for up to one year.
Begin with a 10 µM EdU concentration and adjust as needed for the specific cell type.
The YF® 647A Azide dye has an excitation of 650 nm and emission of 670 nm.
Wear a lab coat and disposable gloves, and handle all reagents in a fume hood or safe environment.