Instruction manual for the TVYBIOTECH SINGLEC3 ELISA kit, covering components, sample handling, assay steps, and performance specifications.
The TVYBIOTECH SINGLEC3 ELISA kit uses a sandwich‑type assay to quantitatively detect Mouse SIGLEC3 protein in serum, plasma, urine or other biological fluids. The kit includes a precoated 96‑well plate, standards, detection reagents, substrate, stop solution and a detailed protocol.
The manual provides guidance on sample collection, reagent preparation, assay workflow, data analysis, and quality‑control criteria such as detection range, sensitivity, specificity, precision, repeatability, stability, and shelf‑life. Proper storage and safety precautions are also described.
| Detection range | 0.16 – 10 ng/mL |
|---|---|
| Intra‑batch precision (CV) | < 10% |
| Inter‑batch precision (CV) | < 12% |
| Stability at 37 °C | 3 days |
| Shelf life | 6 months |
The kit can be used with mouse serum, plasma, urine and other biological fluids, provided the samples are processed as described.
Store the kit at 4 °C for up to one week or at –20 °C for up to six months; avoid repeated freeze‑thaw cycles.
Incubate the plate at 37 °C during the standard and detection steps as specified in the protocol.
The stop solution contains dilute sulfuric acid and is mildly corrosive; handle with appropriate protective equipment.