This manual provides instructions for using the STEMCELL Technologies mTeSR1 kit to culture human induced pluripotent stem cells (hiPS).
The document is a cell culture instruction sheet from the Chinese Academy of Sciences Cell Bank, detailing the use of the STEMCELL Technologies mTeSR1 media kit (Catalog #85850) for maintaining human iPS cells. It outlines specific protocols for reagent preparation, including mixing the 5X additive with base medium and preparing Matrigel coating solutions.
Detailed procedures are provided for cell thawing, passaging, and cryopreservation. The guide specifies conditions such as incubation at 37°C with 5% CO2, media change frequencies, and the use of ROCK inhibitor (Y27632) during specific steps to improve colony survival rates. This manual is written in Chinese.
| Catalog Number | #85850 |
|---|---|
| Base Medium Volume | 400 mL |
| Additive Concentration | 5X |
| Storage Temperature (Base) | 2-8°C |
| Storage Temperature (Additive) | -20°C |
| Recommended Passage Ratio | 1:4 |
The 5X additive should be stored at -20°C and must be used within 3 months after freezing.
Cells should be cultured at 37°C, with 5% CO2 and 95% humidity.