This manual provides detailed instructions for the culture, thawing, subculturing, and cryopreservation of STEM CELL Technologies' H9 Feeder Free human…
This document serves as a user guide for maintaining and culturing H9 Feeder Free human embryonic stem cells. It details the specific reagents required, including mTeSR1 complete medium and BD Matrigel hESC-qualified Matrix, along with their preparation methods.
The manual covers essential laboratory procedures such as cell thawing from cryostorage, routine subculturing to maintain colony density, and cryopreservation protocols. It also includes a troubleshooting section addressing common issues like poor attachment or contamination. This manual is written in Chinese.
| Species | Human |
|---|---|
| Tissue Source | Human Embryonic Inner Cell Mass |
| Morphology | Spherical Clonal |
| Culture Properties | Adherent |
| Safety Level | BSL-2 (Potential Biohazard) |
The cells should be cultured using mTeSR1 complete medium.
The cells require a temperature of 37°C with a gas phase of 95% air and 5% carbon dioxide.
Subculture when colonies become large, the center becomes dense and bright compared to the edge, and adjacent colonies begin to fuse.
The general subculture ratio is between 1:3 and 1:6.