This manual provides instructions for the setup, operation, calibration, and maintenance of the Sony MA900 Cell Sorter, including software usage and aerosol…
The Sony MA900 Cell Sorter is a laboratory instrument used for sorting cells. This document outlines the startup procedures, including the preparation of the instrument with specific chips (70, 100, or 130 µm), cleaning of sample and sort chambers, and filling of fluidic tanks with sheath fluid, waste bleach, and water. It details the initial setup process involving the Cell Sorter Software, automated alignment, and autocalibration using Sony beads to ensure proper laser alignment and stream profile.
| Chip sizes | 70, 100, 130 µm |
|---|---|
| Mandatory laser | 488 nm |
| Autocalibration time | 30 -40 minutes |
| Chip validity | 24h |
| Sample pressure limit | 6 |
The unit is only activated to clear aerosols for 30sec -1 minute before opening the collection chamber and is off during sorting.
The entire process takes approximately 30 minutes, with the first step taking about 10 minutes.
Purity or Semi-Purity is recommended for sorting.