The Shuangluoxuan SM-011203M-A02 PCR kit manual details reagents, equipment, sample prep, PCR conditions, electrophoresis and result interpretation for…
This manual describes the SM-011203M-A02 kit for detecting Shiga toxin‑producing Escherichia coli in food, feed and other samples. It lists the 96‑test composition, each reaction tube containing 12 reagents (23 µL per well) and the required controls and loading buffer.
The procedure covers sample preparation, PCR setup (95 °C 10 min, 30 cycles of 95 °C 30 s, 63 °C 30 s, 72 °C 90 s, final 72 °C 5 min), electrophoresis conditions, and interpretation of results by band pattern or Ct value (≤30 = positive). Precautions for contamination control and reagent handling are also provided. This manual is written in Chinese.
| Detection limit | 10³ CFU/ml |
|---|
The kit can detect as low as 10³ CFU per milliliter of sample.
A sample is considered positive if the electrophoresis shows a distinct band at the target position, or if the fluorescence PCR Ct value is ≤30.
A PCR thermal cycler (e.g., ABI, BioRad, TaKaRa), an electrophoresis apparatus, and a gel imaging system are needed; fluorescence detection can omit electrophoresis.