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Shuangluoxuan SM-011203M-A02 E. coli Shiga Toxin Identification Kit PCR User Manual
This manual describes the SM-011203M-A02 kit for detecting Shiga toxin‑producing Escherichia coli in food, feed and other samples. It lists the 96‑test composition, each reaction tube containing 12 reagents (23 µL per well) and the required controls and loading buffer.
The procedure covers sample preparation, PCR setup (95 °C 10 min, 30 cycles of 95 °C 30 s, 63 °C 30 s, 72 °C 90 s, final 72 °C 5 min), electrophoresis conditions, and interpretation of results by band pattern or Ct value (≤30 = positive). Precautions for contamination control and reagent handling are also provided. This manual is written in Chinese.
Covers: Product Description · Product Composition · Required Instruments · Supplies and Equipment · Sample Handling · Experimental Procedure
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Shuangluoxuan SM-011203M-A02 E. coli Shiga Toxin Identification Kit PCR User Manual
User Manual 3 pages · 307 KB · ZHThe Shuangluoxuan SM-011203M-A02 PCR kit manual details reagents, equipment, sample prep, PCR conditions, electrophoresis and result interpretation for…
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User Manual
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Questions answered in 双螺旋 manuals
What is the detection limit of the SM-011203M-A02 kit?
The kit can detect as low as 10³ CFU per milliliter of sample.
From: Shuangluoxuan SM-011203M-A02 E. coli Shiga Toxin Identification Kit PCR User Manual
How are positive results determined in the PCR assay?
A sample is considered positive if the electrophoresis shows a distinct band at the target position, or if the fluorescence PCR Ct value is ≤30.
From: Shuangluoxuan SM-011203M-A02 E. coli Shiga Toxin Identification Kit PCR User Manual