The SEB SEB083Hu ELISA kit manual provides step‑by‑step instructions for measuring human SOD2 with a sandwich immunoassay.
This manual describes the intended use, reagents, storage conditions, sample handling, and detailed assay procedure for the SEB083Hu 96‑test ELISA kit that quantifies mitochondrial Superoxide Dismutase 2 (SOD2) in human biological samples. It also includes calculation methods, performance specifications, and troubleshooting guidance.
The kit contains a pre‑coated 96‑well strip plate, standards, detection reagents, substrate, stop solution, and wash buffer. Users must follow the described incubation times, washing steps, and reading conditions (450 ± 10 nm) to obtain reliable results.
| Detection range | 0.78‑50 ng/mL |
|---|---|
| Sensitivity (LLD) | < 0.37 ng/mL |
| Recommended microplate reader | Bandwidth ≤ 10 nm, OD range 0‑3 at 450 ± 10 nm |
| Unopened kit storage temperature | -20 °C for plate, 4 °C for other reagents |
| Opened kit usage window | Use remaining reagents within 1 month |
The kit is a sandwich enzyme immunoassay for in‑vitro quantitative measurement of SOD2 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Unopened reagents such as the pre‑coated plate, standards and detection reagents should be stored at –20 °C, while other components are stored at 4 °C. After opening, keep reagents at the same temperatures and reseal the foil pouch; use within one month.
The assay can detect SOD2 concentrations from 0.78 ng/mL up to 50 ng/mL.