The NEB pGLuc Mini-TK 2 Vector is a cloning plasmid for mammalian cells with a minimal HSV TK promoter and secreted Gaussia luciferase reporter.
The pGLuc Mini-TK 2 Vector (catalog N8086S) is a 5,028‑bp plasmid designed for mammalian cell transfection. It contains a minimal Herpes simplex virus thymidine kinase promoter upstream of a Gaussia luciferase coding sequence, a neomycin resistance cassette driven by an SV40 promoter, and standard bacterial replication elements.
The vector supports stable cell line generation, co‑expression with other secreted reporters, and high‑sensitivity luciferase assays. Frequently asked questions cover sequence access, stable line selection, transfection methods, and recommended assay kits.
| Polylinker (MCS) length | 12–68 bp |
|---|---|
| Minimal TK promoter region | 69–131 bp |
| GLuc coding region | 146–703 bp |
| Start codon position | 146–148 bp |
| Stop codon position | 701–703 bp |
| Signal peptide region | 146–196 bp |
| Synthetic poly‑A site | 712–760 bp |
| Neo promoter (SV40) | 1346‑1681 bp |
| Neomycin resistance gene | 1733‑2527 bp |
| Bacterial replication origin (pMB1) | 3861‑3273 bp |
| Ampicillin resistance gene | 4892‑4032 bp |
The sequences of all the plasmids sold by NEB are available online at the NEB technical reference website.
Yes. Selection for neomycin‑resistant colonies can be performed by growing the cells in media containing G418.
Both the BioLux® Gaussia Luciferase Assay Kit (NEB #E3300) and the BioLux Gaussia Luciferase Flex Assay Kit (NEB #E3308) can be used.