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- 2026-09-30
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NEB pGLuc Mini-TK 2 Vector Product Leaflet
The pGLuc Mini-TK 2 Vector (catalog N8086S) is a 5,028‑bp plasmid designed for mammalian cell transfection. It contains a minimal Herpes simplex virus thymidine kinase promoter upstream of a Gaussia luciferase coding sequence, a neomycin resistance cassette driven by an SV40 promoter, and standard bacterial replication elements.
The vector supports stable cell line generation, co‑expression with other secreted reporters, and high‑sensitivity luciferase assays. Frequently asked questions cover sequence access, stable line selection, transfection methods, and recommended assay kits.
Covers: Certificate of Analysis · Features · Recommended Sequencing Primers · Frequently Asked Questions · Companion Products · License and Patents
Latest NEB manuals
NEB pGLuc Mini-TK 2 Vector Product Leaflet
Product Leaflet 2 pages · 373 KB · ENThe NEB pGLuc Mini-TK 2 Vector is a cloning plasmid for mammalian cells with a minimal HSV TK promoter and secreted Gaussia luciferase reporter.
Questions answered in NEB manuals
Where can I find the sequence of this plasmid?
The sequences of all the plasmids sold by NEB are available online at the NEB technical reference website.
Can I generate a stable cell line with pGLuc Mini-TK 2 Vector?
Yes. Selection for neomycin‑resistant colonies can be performed by growing the cells in media containing G418.