User manual for MonAmp SYBR Green qPCR Mix reagents (None/Low/High ROX) by Monad Biotech, covering storage, reaction setup, and troubleshooting.
This manual provides instructions for the MonAmp SYBR Green qPCR Mix, a 2x premixed reagent for SYBR Green I dye-based quantitative PCR. The product includes an antibody-modified hot-start Taq DNA polymerase and optimized buffers to ensure high specificity and amplification efficiency. It is available in three variants: None ROX (REF: MQ10101), Low ROX (REF: MQ10201), and High ROX (REF: MQ10301). This manual is written in Chinese.
| Core Enzyme | Antibody-modified hot-start Taq DNA polymerase |
|---|---|
| Storage Temperature | -20°C |
| Stability after Thawing | 1 month at 4°C |
| Recommended Primer Concentration | 0.2 μM |
| Recommended Template Volume | 1~2 μl |
| Recommended Product Length | 80~200 bp |
It is compatible with Monad q225, q225MX, Bio-Rad CFX series, Roche LightCycler series, Eppendorf Mastercycler ep realplex series, Qiagen/Corbett Rotor-Gene series, Takara Thermal Cycler Dice, and analytikjena qTOWER series.
It is compatible with ABI 7500/7500 Fast, ABI ViiA 7, ABI QuantStudio series, and Stratagene Mx3000P/3005P/4000.
It is compatible with ABI 7000/7300/7700/7900 HT/7900 HT Fast, StepOne, and StepOne Plus.
Store long-term at -20°C in the dark. After thawing, it can be stored at 4°C in the dark for one month.