User manual for JNC's Cellufine GH-25_V6_C chromatography media, covering operation, filling, regeneration, and storage procedures.
This document provides detailed instructions for using Cellufine GH-25_V6_C, a cellulose-based chromatography medium for rapid desalting and buffer exchange. It covers preparation, column packing, operation, regeneration, and storage. The medium is stable across pH 1-14 and compatible with most solvents. Filling procedures include slurry preparation, column assembly, and pressure-controlled filling. Operation involves equilibration, sample loading, washing, and elution. Regeneration uses NaOH wash and buffer rinsing. Storage is recommended at 2-8°C in 20% ethanol suspension.
| Particle Size | 40 - 130 µm (ca. 90 µm) |
|---|---|
| Exclusion Limit Molecular Weight (kD) | 3 |
| Recommended Operating Pressure | <0.2 MPa |
| pH Stability | 1 - 14 |
| Storage Method | 2-8 ºC in 20 % ethanol suspension |
The recommended operating pressure is less than 0.2 MPa.
The exclusion limit molecular weight is 3 kD.
Store at 2-8°C in a 20% ethanol suspension.
The recommended linear flow rate is 100-300 cm/h.