Instruction manual for Geruisi Bio's G0558W GlmS enzyme activity assay kit, detailing reagents, protocol, and data calculation.
This manual describes the 6‑phosphoglucosamine synthase (GlmS) activity assay kit, including the principle of the enzymatic reaction and the detection of glutamate production. It lists all reagents, their quantities, storage conditions, and the required laboratory equipment.
Step‑by‑step procedures cover sample preparation for tissue, bacterial or cell extracts, the incubation conditions, color development, and the calculation of enzyme activity using the provided standard curve equation. This manual is written in Chinese.
| Extraction solution volume per vial | 60 mL |
|---|---|
| Reagent 2 liquid volume per vial | 25 mL |
| Standard curve equation | y = 9.4636x – 0.0018 |
The microplate reader is set to a wavelength of 450 nm.
The reaction is incubated at 37 °C for 30 minutes.
Approximately 0.1 g of tissue is mixed with 1 mL of extraction solution.