Instructions for using the Geruisi Bio G0206F reduced glutathione (GSH) assay kit to measure intracellular GSH levels via spectrophotometry.
The G0206F assay kit from Geruisi Bio measures reduced glutathione (GSH) in cells, tissues, bacteria, or liquid samples. GSH reacts with DTNB to produce a complex that absorbs at 412 nm, and the absorbance is proportional to GSH concentration.
The manual details reagent components, required instruments (visible spectrophotometer, 1 mL cuvettes, centrifuge, water bath, pipettes), sample preparation steps for tissue, bacterial/cell, and liquid samples, the measurement procedure, and calculations for GSH concentration using the provided standard curve. This manual is written in Chinese.
| Number of samples per kit | 48 |
|---|---|
| Measurement wavelength | 412 nm |
| Standard curve equation | y = 1.5403x + 0.0049 |
| Reagent 1 volume per assay | 480 µL |
| Reagent 2 volume per assay | 160 µL |
Set the spectrophotometer to 412 nm.
ΔA is the absorbance of the test tube minus the absorbance of the blank tube (ΔA = A_test – A_blank).
A visible spectrophotometer, 1 mL glass cuvettes (1 cm path length), a low‑temperature centrifuge, a water bath, and adjustable pipettes.
The standard curve is y = 1.5403x + 0.0049, where y is ΔA and x is the standard concentration (µmol/mL).