Instruction manual for Foregene RTEasyTMI MasterMix (Cat.No. RT‑01011/01012) covering product features, storage, preparation and troubleshooting.
The Foregene RTEasyTMI MasterMix is a reverse‑transcription reagent kit designed for fast, high‑sensitivity synthesis of first‑strand cDNA from pg‑level RNA. The manual details product introduction, key features such as high alcohol (45 %) and guanidine salt (750 mM) tolerance, and a range of applications including RT‑PCR, cDNA cloning and RACE.
The guide provides step‑by‑step instructions for reagent preparation, reaction setup, recommended temperatures (up to 50 °C) and times (25 min), storage conditions (‑20 °C), safety precautions, and a troubleshooting section to address common issues like non‑specific amplification or lack of signal.
| Alcohol tolerance | 45 % |
|---|---|
| Guanidine salt tolerance | 750 mM |
| Maximum reaction temperature | 50 °C |
| Typical RT reaction time | 25 min |
| Storage temperature | -20 °C |
The reverse transcription can be completed in about 25 minutes.
The kit tolerates up to 45 % alcohol and up to 750 mM guanidine salt in the RNA sample.
For complex secondary‑structure RNA, a reaction temperature of 50 °C is recommended.