Instruction manual for FOREGENE PlantSeedDirectPCRKit I/II, a direct PCR kit for plant seeds.
The FOREGENE PlantSeedDirectPCRKit I/II provides a rapid lysis system that releases genomic DNA from low‑polysaccharide, low‑polyphenol plant seeds for PCR without prior DNA purification. The kit includes buffers, a 2×SeedPCREasyTMMix, loading buffer and detailed instructions for sample preparation, lysis, and PCR setup.
The manual covers product features, storage conditions, safety precautions, step‑by‑step operation, and troubleshooting guidance. It also lists the kit contents for different reaction scales and provides recommended equipment and reagents for successful direct PCR experiments. This manual is written in Chinese.
| DNA release time | 5‑10 min |
|---|---|
| Maximum amplicon size | ≤1 kb |
| BufferSP1 storage life | 12 months dry, 2‑8 °C recommended |
| 2×SeedPCREasyTMMix storage temperature | -20 °C (avoid freeze‑thaw) |
The kit can reliably amplify fragments up to 1 kb; larger fragments may have reduced efficiency or fail.
Store BufferSP1 dry; it remains stable for 12 months at 2‑8 °C. Low‑temperature storage can cause precipitation, so avoid storing it below 4 °C.
The 2×SeedPCREasyTMMix should be stored at –20 °C and protected from repeated freeze‑thaw cycles.