The Exongen A602 AccuProbe qPCR Master Mix User Manual provides composition, storage, recommended PCR conditions, and usage guidelines for researchers.
The AccuProbe qPCR Master Mix A602 is a 2× premix reagent designed for probe‑based real‑time PCR. It contains a chemically modified hot‑start DNA polymerase, UDG enzyme, dNTPs, dUTP, Mg and optional 50× ROX Reference Dye. The product is supplied in a 5 ml vial (A602‑01) and should be stored at –20 °C, protected from light, for up to two years.
The manual details how to set up a 20 µl reaction by adding template, primers, probe, optional ROX dye and water to achieve a 1× working concentration. Recommended PCR cycling steps, annealing/extension temperatures, and instrument‑specific ROX requirements are listed. Precautions include avoiding repeated freeze‑thaw cycles and mixing the premix by inversion rather than vortexing. This manual is written in Chinese.
| Product code | A602 |
|---|---|
| Storage temperature | -20 °C, protected from light, shelf life two years |
| Components | 2×AccuProbe qPCR Master Mix, 50×ROX Reference Dye I & II |
Store the reagent at –20 °C, protected from light, and it remains stable for up to two years.